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viral np protein  (Sino Biological)


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    Structured Review

    Sino Biological viral np protein
    Viral Np Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleoprotein+np/Influenza+A+H1N1+(A%2FPuerto+Rico%2F8%2F34%2FMount+Sinai)+Nucleoprotein+%2F+NP+(I116M)+Protein/pm42049736-300-12-15
    Average 95 stars, based on 61 article reviews
    viral np protein - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Infection:

    Article Title: Specific Monoclonal Antibodies Targeting Unique HA Epitopes Block H7N9 Influenza A Viral Replication.
    Article Snippet: .. Viral infection was quantified by indirect ELISA with MAbs against the nucleoprotein (NP) of influenza A virus (Sino Biological). .. The final concentration of antibody that reduced infection to 50% (IC50) was determined using GraphPad Prism 6 software.

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
    Article Snippet: .. After 20h viral infection cells was quantified by indirect ELISA with nanobodies against the nucleoprotein (NP) of influenza A virus (Sino Biological, 11675-V08B). .. The final concentration of nanobody that reduced infection to 50% (IC 50 ) was determined using GraphPad Prism 6 software.

    Indirect ELISA:

    Article Title: Specific Monoclonal Antibodies Targeting Unique HA Epitopes Block H7N9 Influenza A Viral Replication.
    Article Snippet: .. Viral infection was quantified by indirect ELISA with MAbs against the nucleoprotein (NP) of influenza A virus (Sino Biological). .. The final concentration of antibody that reduced infection to 50% (IC50) was determined using GraphPad Prism 6 software.

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
    Article Snippet: .. After 20h viral infection cells was quantified by indirect ELISA with nanobodies against the nucleoprotein (NP) of influenza A virus (Sino Biological, 11675-V08B). .. The final concentration of nanobody that reduced infection to 50% (IC 50 ) was determined using GraphPad Prism 6 software.

    Virus:

    Article Title: Specific Monoclonal Antibodies Targeting Unique HA Epitopes Block H7N9 Influenza A Viral Replication.
    Article Snippet: .. Viral infection was quantified by indirect ELISA with MAbs against the nucleoprotein (NP) of influenza A virus (Sino Biological). .. The final concentration of antibody that reduced infection to 50% (IC50) was determined using GraphPad Prism 6 software.

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
    Article Snippet: .. After 20h viral infection cells was quantified by indirect ELISA with nanobodies against the nucleoprotein (NP) of influenza A virus (Sino Biological, 11675-V08B). .. The final concentration of nanobody that reduced infection to 50% (IC 50 ) was determined using GraphPad Prism 6 software.

    Binding Assay:

    Article Title: Broad spectrum influenza virus vaccine
    Article Snippet: .. 11 Naïve 0 μg None None To test the sera for the presence of antibodies capable of binding to hemagglutinin (HA) from a wide variety of influenza strains or nucleoprotein (NP), ELISA plates were coated with 100 ng of the following recombinant proteins obtained from Sino Biological Inc.: Influenza A H1N1 (A/New Caledonia/20/99) HA, cat #11683-V08H; Influenza A H3N2 (A/Aichi/2/1968) HA, cat #11707-V08H; Influenza A H1N1 (A/California/04/2009) HA, cat #11055-V08H; Influenza A H1N1 (A/Puerto Rico/8/34) HA, cat #11684-V08H; Influenza A H1N (A/Brisbane/59/2007) HA, cat #11052-V08H; Influenza A H2N2 (A/Japan/305/1957) HA, cat #11088-V08H; Influenza A H7N9 (A/Anhui/1/2013) HA, cat #40103-V08H, Influenza A H3N2 (A/Moscow/10/99) HA, cat #40154-V08 and Influenza A H3N2 (A/Aichi/2/1968) Nucleoprotein cat #40207-V08B. ..

    Article Title: Evaluation of Antibody-Dependent Fc-Mediated Viral Entry, as Compared With Neutralization, in SARS-CoV-2 Infection.
    Article Snippet: .. Seven SARS-CoV-2 antigens were used to profile antibody responses, including S extra-cellular domain (S ECD), which comprises the subunit 1 (S1) and 2 (S2) (S1+S2 ECD), S1, S2, the nucleoprotein (NP) (Sino Biological,; 40589-V08B1, 40591-V08B1, 40590-V08B and 40588-V08B), the S Receptor Binding Domain (RBD), S trimer (LakePharma; 46438 and 46328) and S N Terminal Domain [NTD; produced in house (78)]. .. All antigens corresponded to the SARS-CoV-2 Wuhan-Hu-1 strain.

    Article Title: Evaluation of Antibody-Dependent Fc-Mediated Viral Entry, as Compared With Neutralization, in SARS-CoV-2 Infection
    Article Snippet: FVE was calculated as the fold increase in viral entry in the presence of plasma; curves were analyzed using GraphPad Prism to determine the area under the curve >1 (FVE AUC), maximum fold increase in viral entry (max FVE), and the plasma dilution at which maximum viral entry was observed. .. Seven SARS-CoV-2 antigens were used to profile antibody responses, including S extra-cellular domain (S ECD), which comprises the subunit 1 (S1) and 2 (S2) (S1+S2 ECD), S1, S2, the nucleoprotein (NP) (Sino Biological,; 40589-V08B1, 40591-V08B1, 40590-V08B and 40588-V08B), the S Receptor Binding Domain (RBD), S trimer (LakePharma; 46438 and 46328) and S N Terminal Domain [NTD; produced in house ( )]. .. All antigens corresponded to the SARS-CoV-2 Wuhan-Hu-1 strain.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Broad spectrum influenza virus vaccine
    Article Snippet: .. 11 Naïve 0 μg None None To test the sera for the presence of antibodies capable of binding to hemagglutinin (HA) from a wide variety of influenza strains or nucleoprotein (NP), ELISA plates were coated with 100 ng of the following recombinant proteins obtained from Sino Biological Inc.: Influenza A H1N1 (A/New Caledonia/20/99) HA, cat #11683-V08H; Influenza A H3N2 (A/Aichi/2/1968) HA, cat #11707-V08H; Influenza A H1N1 (A/California/04/2009) HA, cat #11055-V08H; Influenza A H1N1 (A/Puerto Rico/8/34) HA, cat #11684-V08H; Influenza A H1N (A/Brisbane/59/2007) HA, cat #11052-V08H; Influenza A H2N2 (A/Japan/305/1957) HA, cat #11088-V08H; Influenza A H7N9 (A/Anhui/1/2013) HA, cat #40103-V08H, Influenza A H3N2 (A/Moscow/10/99) HA, cat #40154-V08 and Influenza A H3N2 (A/Aichi/2/1968) Nucleoprotein cat #40207-V08B. ..

    Recombinant:

    Article Title: Broad spectrum influenza virus vaccine
    Article Snippet: .. 11 Naïve 0 μg None None To test the sera for the presence of antibodies capable of binding to hemagglutinin (HA) from a wide variety of influenza strains or nucleoprotein (NP), ELISA plates were coated with 100 ng of the following recombinant proteins obtained from Sino Biological Inc.: Influenza A H1N1 (A/New Caledonia/20/99) HA, cat #11683-V08H; Influenza A H3N2 (A/Aichi/2/1968) HA, cat #11707-V08H; Influenza A H1N1 (A/California/04/2009) HA, cat #11055-V08H; Influenza A H1N1 (A/Puerto Rico/8/34) HA, cat #11684-V08H; Influenza A H1N (A/Brisbane/59/2007) HA, cat #11052-V08H; Influenza A H2N2 (A/Japan/305/1957) HA, cat #11088-V08H; Influenza A H7N9 (A/Anhui/1/2013) HA, cat #40103-V08H, Influenza A H3N2 (A/Moscow/10/99) HA, cat #40154-V08 and Influenza A H3N2 (A/Aichi/2/1968) Nucleoprotein cat #40207-V08B. ..

    Article Title: Safety and Biocompatibility of a Spray-Dried Influenza Microparticle Vaccine in Mice
    Article Snippet: Acetic acid, acetic anhydride, acetone, acetonitrile, alphabromoisobutyryl bromide, basic alumina powder, chloroform, copper acetate monohydrate, dimethyl formamide, ethyl ether, hexane, magnesium sulfate, methylene chloride, n-propylamine, pentane, petroleum ether, Pluronic® F127, potassium carbonate, sea sand, sodium borohydride, sodium hydroxide, sulfuric acid, triethylamine, and toluene were purchased from Fisher Scientific (Waltham, MA). .. Recombinant influenza A/HK/1/68 nucleoprotein (NP) was acquired from Sino Biological (Beijing, China). .. CpG ODN 1668 and cyclic dinucleotide (CDN) di-guanosine monophosphate were purchased from InvivoGen (San Diego, CA).

    Article Title: Equine influenza antigen-based particle vaccine protects mice against homologous and heterosubtypic viral challenges.
    Article Snippet: Influenza virus is a common source of respiratory illness and poses a major public health burden globally.. Significant efforts have been dedicated towards developing and deploying effective vaccines.. However, since being licensed in 1945, influenza vaccines have made limited progress and still suffer from several limitations, including suboptimal efficacy against variants.

    Produced:

    Article Title: Evaluation of Antibody-Dependent Fc-Mediated Viral Entry, as Compared With Neutralization, in SARS-CoV-2 Infection.
    Article Snippet: .. Seven SARS-CoV-2 antigens were used to profile antibody responses, including S extra-cellular domain (S ECD), which comprises the subunit 1 (S1) and 2 (S2) (S1+S2 ECD), S1, S2, the nucleoprotein (NP) (Sino Biological,; 40589-V08B1, 40591-V08B1, 40590-V08B and 40588-V08B), the S Receptor Binding Domain (RBD), S trimer (LakePharma; 46438 and 46328) and S N Terminal Domain [NTD; produced in house (78)]. .. All antigens corresponded to the SARS-CoV-2 Wuhan-Hu-1 strain.

    Article Title: Evaluation of Antibody-Dependent Fc-Mediated Viral Entry, as Compared With Neutralization, in SARS-CoV-2 Infection
    Article Snippet: FVE was calculated as the fold increase in viral entry in the presence of plasma; curves were analyzed using GraphPad Prism to determine the area under the curve >1 (FVE AUC), maximum fold increase in viral entry (max FVE), and the plasma dilution at which maximum viral entry was observed. .. Seven SARS-CoV-2 antigens were used to profile antibody responses, including S extra-cellular domain (S ECD), which comprises the subunit 1 (S1) and 2 (S2) (S1+S2 ECD), S1, S2, the nucleoprotein (NP) (Sino Biological,; 40589-V08B1, 40591-V08B1, 40590-V08B and 40588-V08B), the S Receptor Binding Domain (RBD), S trimer (LakePharma; 46438 and 46328) and S N Terminal Domain [NTD; produced in house ( )]. .. All antigens corresponded to the SARS-CoV-2 Wuhan-Hu-1 strain.

    Immunohistochemistry:

    Article Title: Agreement and differential use of laboratory methods for the detection and quantification of SARS-CoV-2 in experimentally infected animals
    Article Snippet: .. IHC was performed on formalin-fixed tissue slides using a Nucleoprotein (NP)-specific monoclonal primary antibody (Sino Biological, ref. 40143-R019; ; ). .. Viral titration by CPE assay was performed in vitro on Vero-E6 cells (ATCC CRL-1587) and the median tissue culture infectious dose (TCID 50 /ml) was calculated with the Reed-Muench method ( ).



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    (A) Cell viability was assessed by CCK-8 assay following T5224 treatment. For infection experiments, cells were pretreated with 0, 10, or 20 μM T5224 for 2 h before PR8 virus infection. Protein lysates were harvested at 24 h post-infection and analyzed by Western blot <t>using</t> <t>anti-NP</t> and anti-GAPDH antibodies. (B) 293H cells were co-transfection with M2 and c-Fos plasmids, alongside M2 and vector controls. After 48 h, cells were lysed and RNA was extracted for RT-qPCR analysis of M2 mRNA expression. (C and D) Co-immunoprecipitation and immunoblot analysis of 293H cells co-transfected with Flag-c-Fos and M2 (C) or NP (D). (E) 293H cells were co-transfection with Flag-c-Fos and M2 for 48 h, followed by immunostaining for Flag (red) and M2 (green). Representative confocal immunofluorescence images are shown. Scale bars, 2 μm. (F) 293H cells were co-transfected with M2 and Flag-c-Fos or empty vector. Cells were treated with 50 μg/mL CHX and harvested at the indicated time points (0 - 8 h). M2 protein levels were analyzed by Western blot. (G and H) 293H cells were transfected with M2 or empty vector. At 6 h post-transfection, cells were treated with MG132 (100, 200, 400 nM), Bafilomycin A1 (1, 2, 5 nM), or DMSO control. After 48 h, M2 protein expression was assessed by Western blot. (I) 293H cells were co-transfected with M2 and Flag-c-Fos or vector, followed by treatment with MG132 (200 nM) or Bafilomycin A1 (2 nM) for 48 h. M2 protein levels were analyzed by Western blot. All data are presented as means ± SD from at least three independent experiments. Significance was determined by Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. #, P > 0.05.
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    (A) Cell viability was assessed by CCK-8 assay following T5224 treatment. For infection experiments, cells were pretreated with 0, 10, or 20 μM T5224 for 2 h before PR8 virus infection. Protein lysates were harvested at 24 h post-infection and analyzed by Western blot <t>using</t> <t>anti-NP</t> and anti-GAPDH antibodies. (B) 293H cells were co-transfection with M2 and c-Fos plasmids, alongside M2 and vector controls. After 48 h, cells were lysed and RNA was extracted for RT-qPCR analysis of M2 mRNA expression. (C and D) Co-immunoprecipitation and immunoblot analysis of 293H cells co-transfected with Flag-c-Fos and M2 (C) or NP (D). (E) 293H cells were co-transfection with Flag-c-Fos and M2 for 48 h, followed by immunostaining for Flag (red) and M2 (green). Representative confocal immunofluorescence images are shown. Scale bars, 2 μm. (F) 293H cells were co-transfected with M2 and Flag-c-Fos or empty vector. Cells were treated with 50 μg/mL CHX and harvested at the indicated time points (0 - 8 h). M2 protein levels were analyzed by Western blot. (G and H) 293H cells were transfected with M2 or empty vector. At 6 h post-transfection, cells were treated with MG132 (100, 200, 400 nM), Bafilomycin A1 (1, 2, 5 nM), or DMSO control. After 48 h, M2 protein expression was assessed by Western blot. (I) 293H cells were co-transfected with M2 and Flag-c-Fos or vector, followed by treatment with MG132 (200 nM) or Bafilomycin A1 (2 nM) for 48 h. M2 protein levels were analyzed by Western blot. All data are presented as means ± SD from at least three independent experiments. Significance was determined by Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. #, P > 0.05.
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    (A) Cell viability was assessed by CCK-8 assay following T5224 treatment. For infection experiments, cells were pretreated with 0, 10, or 20 μM T5224 for 2 h before PR8 virus infection. Protein lysates were harvested at 24 h post-infection and analyzed by Western blot <t>using</t> <t>anti-NP</t> and anti-GAPDH antibodies. (B) 293H cells were co-transfection with M2 and c-Fos plasmids, alongside M2 and vector controls. After 48 h, cells were lysed and RNA was extracted for RT-qPCR analysis of M2 mRNA expression. (C and D) Co-immunoprecipitation and immunoblot analysis of 293H cells co-transfected with Flag-c-Fos and M2 (C) or NP (D). (E) 293H cells were co-transfection with Flag-c-Fos and M2 for 48 h, followed by immunostaining for Flag (red) and M2 (green). Representative confocal immunofluorescence images are shown. Scale bars, 2 μm. (F) 293H cells were co-transfected with M2 and Flag-c-Fos or empty vector. Cells were treated with 50 μg/mL CHX and harvested at the indicated time points (0 - 8 h). M2 protein levels were analyzed by Western blot. (G and H) 293H cells were transfected with M2 or empty vector. At 6 h post-transfection, cells were treated with MG132 (100, 200, 400 nM), Bafilomycin A1 (1, 2, 5 nM), or DMSO control. After 48 h, M2 protein expression was assessed by Western blot. (I) 293H cells were co-transfected with M2 and Flag-c-Fos or vector, followed by treatment with MG132 (200 nM) or Bafilomycin A1 (2 nM) for 48 h. M2 protein levels were analyzed by Western blot. All data are presented as means ± SD from at least three independent experiments. Significance was determined by Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. #, P > 0.05.
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    (A) Cell viability was assessed by CCK-8 assay following T5224 treatment. For infection experiments, cells were pretreated with 0, 10, or 20 μM T5224 for 2 h before PR8 virus infection. Protein lysates were harvested at 24 h post-infection and analyzed by Western blot <t>using</t> <t>anti-NP</t> and anti-GAPDH antibodies. (B) 293H cells were co-transfection with M2 and c-Fos plasmids, alongside M2 and vector controls. After 48 h, cells were lysed and RNA was extracted for RT-qPCR analysis of M2 mRNA expression. (C and D) Co-immunoprecipitation and immunoblot analysis of 293H cells co-transfected with Flag-c-Fos and M2 (C) or NP (D). (E) 293H cells were co-transfection with Flag-c-Fos and M2 for 48 h, followed by immunostaining for Flag (red) and M2 (green). Representative confocal immunofluorescence images are shown. Scale bars, 2 μm. (F) 293H cells were co-transfected with M2 and Flag-c-Fos or empty vector. Cells were treated with 50 μg/mL CHX and harvested at the indicated time points (0 - 8 h). M2 protein levels were analyzed by Western blot. (G and H) 293H cells were transfected with M2 or empty vector. At 6 h post-transfection, cells were treated with MG132 (100, 200, 400 nM), Bafilomycin A1 (1, 2, 5 nM), or DMSO control. After 48 h, M2 protein expression was assessed by Western blot. (I) 293H cells were co-transfected with M2 and Flag-c-Fos or vector, followed by treatment with MG132 (200 nM) or Bafilomycin A1 (2 nM) for 48 h. M2 protein levels were analyzed by Western blot. All data are presented as means ± SD from at least three independent experiments. Significance was determined by Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. #, P > 0.05.
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    Image Search Results


    (A) Cell viability was assessed by CCK-8 assay following T5224 treatment. For infection experiments, cells were pretreated with 0, 10, or 20 μM T5224 for 2 h before PR8 virus infection. Protein lysates were harvested at 24 h post-infection and analyzed by Western blot using anti-NP and anti-GAPDH antibodies. (B) 293H cells were co-transfection with M2 and c-Fos plasmids, alongside M2 and vector controls. After 48 h, cells were lysed and RNA was extracted for RT-qPCR analysis of M2 mRNA expression. (C and D) Co-immunoprecipitation and immunoblot analysis of 293H cells co-transfected with Flag-c-Fos and M2 (C) or NP (D). (E) 293H cells were co-transfection with Flag-c-Fos and M2 for 48 h, followed by immunostaining for Flag (red) and M2 (green). Representative confocal immunofluorescence images are shown. Scale bars, 2 μm. (F) 293H cells were co-transfected with M2 and Flag-c-Fos or empty vector. Cells were treated with 50 μg/mL CHX and harvested at the indicated time points (0 - 8 h). M2 protein levels were analyzed by Western blot. (G and H) 293H cells were transfected with M2 or empty vector. At 6 h post-transfection, cells were treated with MG132 (100, 200, 400 nM), Bafilomycin A1 (1, 2, 5 nM), or DMSO control. After 48 h, M2 protein expression was assessed by Western blot. (I) 293H cells were co-transfected with M2 and Flag-c-Fos or vector, followed by treatment with MG132 (200 nM) or Bafilomycin A1 (2 nM) for 48 h. M2 protein levels were analyzed by Western blot. All data are presented as means ± SD from at least three independent experiments. Significance was determined by Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. #, P > 0.05.

    Journal: bioRxiv

    Article Title: c-Fos enhances influenza virus replication by stabilizing the M2 protein and promoting autophagosome accumulation

    doi: 10.64898/2026.03.05.709812

    Figure Lengend Snippet: (A) Cell viability was assessed by CCK-8 assay following T5224 treatment. For infection experiments, cells were pretreated with 0, 10, or 20 μM T5224 for 2 h before PR8 virus infection. Protein lysates were harvested at 24 h post-infection and analyzed by Western blot using anti-NP and anti-GAPDH antibodies. (B) 293H cells were co-transfection with M2 and c-Fos plasmids, alongside M2 and vector controls. After 48 h, cells were lysed and RNA was extracted for RT-qPCR analysis of M2 mRNA expression. (C and D) Co-immunoprecipitation and immunoblot analysis of 293H cells co-transfected with Flag-c-Fos and M2 (C) or NP (D). (E) 293H cells were co-transfection with Flag-c-Fos and M2 for 48 h, followed by immunostaining for Flag (red) and M2 (green). Representative confocal immunofluorescence images are shown. Scale bars, 2 μm. (F) 293H cells were co-transfected with M2 and Flag-c-Fos or empty vector. Cells were treated with 50 μg/mL CHX and harvested at the indicated time points (0 - 8 h). M2 protein levels were analyzed by Western blot. (G and H) 293H cells were transfected with M2 or empty vector. At 6 h post-transfection, cells were treated with MG132 (100, 200, 400 nM), Bafilomycin A1 (1, 2, 5 nM), or DMSO control. After 48 h, M2 protein expression was assessed by Western blot. (I) 293H cells were co-transfected with M2 and Flag-c-Fos or vector, followed by treatment with MG132 (200 nM) or Bafilomycin A1 (2 nM) for 48 h. M2 protein levels were analyzed by Western blot. All data are presented as means ± SD from at least three independent experiments. Significance was determined by Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. #, P > 0.05.

    Article Snippet: The following primary antibodies were used: anti-LC3B (2775; Cell Signaling Technology, CST), anti-SQSTM1 (5114; CST), anti-GAPDH (2118; CST), anti-NP (10780-01; SouthernBiotech), anti-SERCA (A1097; ABclonal), anti-Flag (AE092; ABclonal), and anti-influenza A virus M2 (IT-003-015; Immune Technology).

    Techniques: CCK-8 Assay, Infection, Virus, Western Blot, Cotransfection, Plasmid Preparation, Quantitative RT-PCR, Expressing, Immunoprecipitation, Transfection, Immunostaining, Immunofluorescence, Control